Document
FBI Laboratory Examination Reports on Nikumaroro Button Artifact Submitted by TIGHAR
Plain-English summary
TIGHAR submitted a stained button recovered from Nikumaroro island to the FBI Laboratory to determine if organic matter or DNA related to Amelia Earhart's disappearance could be detected. FBI serology testing found no presence of blood on the button. Subsequent mitochondrial DNA testing of a swab from the button yielded a sequence with multiple ambiguous nucleotide positions, making it unsuitable for comparison purposes.
Key line (verbatim from the document)“The sequence from this sample showed the presence of multiple ambiguous nucleotide positions and thus was not suitable for comparison purposes”
Summary written with AI help from the document text and checked against it; the key line is matched word for word to the text. Read the original before citing.
Tags
Amelia EarhartFBI LaboratoryFijiFort Walton Beach, FLGardner IslandGilbert IslandsJames MatthewsKaren BurnsNikumaroroRichard E. GillespieRichard JantzTIGHARThe International Group for Historic Aircraft RecoveryThomas T. KubicWilmington, Delaware
SourceNational Archives (NARA). Original. Released 2025-12-17. U.S. government work, public domain. Text from OCR; may contain errors.
Full text (9,479 characters)
:
Ald SIHL OL GAdGV YO WO¥S GSAOWSY 3¢ LON LSNW VIYSLV
9[1J Siu} Buljpuey ul aleg asp
L2ve leD - sajsuedy
“equinu 9Ng plo ayy AyyUap! oO} seu
yafqns ay} Bulsn | L9ep UOIsUa}xXo [}ed OS|MUaUIO
‘umouy jt “aquinu ajljng pjo ayy Buisn Lzpep
uolsua}xe Huyje9 Aq 91} 4oyJO ay} UleE}qo Ae No,
“Oils SIU} YUM JUap! oq Aew sequunu ayyng Plo ayy
Japun 9ji} Jayjoue yey} seme aq aseajd “aquiny
d]l4 BSED JESIBAIUN OY} JO UONeJUSWa|dU! YA,
S314 SALLWYLSININGY GNV SAILLVDLLSSANI OHIGs
SYHSLUVf |
t
fi
H
S
é 3” —
a a
a NOW! §) 3=:;
\ , B/E 2 =)
Re nvauna > sib? 2
E a ; =
fle sg
la sousnr 4o unz “12 38
q
:
|
|
|
“N ZEL-Ob “AOY $ 965-7
Sa Ea aaa aaa aaa
Y
L
a @
2123 }o |
2812 Fawkes Drive *Wilmington, Delaware 19808 * USA
302) 994-4410 * Fax (302) 994-7945
P Mistorneuer | { o1oz2s012
Thomas T. Kubic
| Dep. Asst. Dir.
| Rm 3012
Criminal Div.
cms -VIO
Siu 6S IMS -V
9th. St. & Pennsylvania Ave. (Vsstu 4 Pe
Wash. DC 20503 Disappeasunce Rory
Pimelra fe Ear hart
Dear Tom,
For the record, TIGHAR (pronounced tiger) is the acronym for The
International Group for Historic Aircraft Recovery. We're a nonprofit 501
(c)(3) tax exempt historical and educational foundation based in
Wilmington, Delaware. Our investigation of the Earhart disappearance
began in 1988.
The enclosed button - TIGHAR Artifact 2-3-W-5 - could be a key piece of
evidence in that investigation.
Our basic question is whether the dark stain on the button's surface
5 might be organic material due to the button having been in contact with
8 decaying flesh. We really have no expectation that DNA can be extracted
- 3 from such a degraded deposit. Besides, the button has been handled by
a . .
= many people since its recovery. _ , , f
Pe CF t+ re Ay 4 ——
58 Yi: Oussorter
Here's a brief rundown on what we know and what we suspect:
The Pacific island where the button was found - Nikumaroro (formerly
known as Gardner Island) - was considered by the U.S. Navy, at the time
of Earhart's disappearance in July 1937, to be the most likely place for
the lost flight to have landed. An aerial search of the atoll one week after
as
Original
Cc
‘Opy & Specs Detacheg
the disappearance revealed "clear signs of recent habitation" but no
airplane or people were seen and the searchers moved on, not realizing
that the island had been uninhabited since 1892.
In 1939 the British set out to colonize the island with natives from the
overpopulated Gilbert Islands. A year later, in 1940, a native work party
cutting trees on a remote part of the island came upon a human skull
which they buried. When a British administrator arrived a few months
later he heard of the discovery and ordered a search of the area. The
search revealed a crude campsite where a castaway had clearly tried, but
failed, to survive. There were some dead birds, a dead turtle, the remains
of a fire, and - under a nearby tree - a partial human skeleton including
the mandible that matched the skull (which was exhumed). Also at the
site were a few artifacts including the remains of a woman's shoe and a
box which was judged to have once contained a sextant. The British
administrator suspected that he had found Amelia Earhart and so
informed his superiors on the island of Fiji.
010223012
The British authorities in Fiji ordered the bones and artifacts sent there
and the entire matter kept secret (we have all of the official British
correspondence on this). The bones were examined and measured by a
physician in Fiji and judged to be those of a "short, stocky European or
mixed-race male." The British dropped the investigation and the matter
was largely forgotten. We don't know what became of the bones but we
do have the measurements taken by the doctor. Three years ago,
forensic anthropologists Karen Burns PhD at the University of Georgia,
Athens; and Richard Jantz PhD at the University of Tennessee, Knoxville
independently evaluated the measurements and concluded that the |
remains were most likely those of a white female of northern European
descent who stood about 5 feet 7 inches tall. That's a pretty good
description of Amelia Earhart.
In five archaeological search expeditions to the island we have found a
number of artifacts which, although none is diagnostic, appear to be
consistent with components of the type of airplane Earhart was flying and
the shoes she is known to have been wearing.
Original
Copy & Specs Detached
The button was found in 1996 in an area we now believe to be that in
which the bones were found in 1940. It seems a bit large for a shirt
button and is more consistent with a trouser button. The native laborers
on the island often wore shorts fastened with buttons, but the British
supply records indicate that the buttons were made of bone.
An examination of this button by archaeologist James Matthews of Fort
Walton Beach, FL concluded, by a “hot needle test”, that it is probably
Bakelite. The button does not seem to match any known WWII military
design. The button may have been on clothing worn by the castaway
whom we know died on the island and whom we suspect died near where
the button was found.
We'll be returning to the island in August of this year for more search
operations. If the dark stain on the button is due to contact with rotting
flesh, the possibility that it is from the dead castaway would seem to
increase. That would justify an intensive search of the area for more
evidence. If we have the right area, there is a hole nearby which just may
be where the skull was dug up and, if so, that hole may still contain a
tooth or two from the skull. As you know, a tooth can be a good source
of mtDNA. Earhart had a sister (now dead) whose daughter (Amelia's
niece) lives in Silverspring, MD, so we do have a source to match against
should we be so fortunate as to recover DNA.
There is, of course, much more to the story. You'll find volumes of
material about our twelve year (to date) investigation on our website at
www.tighar.org.
We really appreciate any help you can provide. Please don't hesitate to
call (320/994-4410) or email ([email protected]) with any questions you
may have.
Sincerely,
Richard E. Gillespie
Executive Director
010223012
yea ,
Y TA Rev. $-13-99)
FEDERAL BUREAU OF INVESTIGATION
WASHINGTON, D. C. 20535
: : . Dae: May 7, 2001
To: Richard E. Gillespie
Executive Director
The Intemational Group for
Historic Aircraft Recovery CaseID No: 95D-HQ-1334954 - 2.
2812 Fawkes Drive
Wilmington, Delaware 19808 LabNo: 010223012 MQ BO
Reference: Your letter dated February 15, 2001
Your No.:
Tide: UNSUB;
AMELIA EARHART - VICTIM;
MISSING PERSONS
Date specimens received: February 23, 2001
Specimens:
Ql Button (your number Artifact 2-3-W-5)
NE1 "TIGHAR TRACKS" Journal, Vol. 15, 1999
The results of the serology examinations are included in this report. The
mitochondrial DNA examinations are continuing and you will subsequently be advised
conceming the results and the disposition of Q1 and NE}.
Page 1 of I
shot aH
MAILROOM ;
This Report is Fumished for Official Use Only
hos ..
oF Tela (Rev. 5-18-99) @ AS
a
LABORATORY
FEDERAL BUREAU OF INVESTIGATION
WASHINGTON, D. C. 20535
Report of. Examination
— | bec May 1, 2001
Unit: DNA Analysis I PhoneNo: 202-324-4409
CaseID No: 95D-HQ-1334954 LabNo: 010223012 MQ BO
Results of Examinations:
Specimen QI was examined for the presence of blood; however, none was found.
No other serological examinations were conducted.
Reviewer: Date: awd: ZO !
DNAU I - Page To
This Report is Fumished for Official Use Only
Pare 9}
7-1 (Rev. 5-13-99)
1
FEDERAL BUREAU OF AURETTeatTTON
WASHINGTON, D. C. 20535
; . : Date: October 24, 2001
To Richard E. Gillespie
Executive Director
The International Group for
Historic Aircraft Recovery Case ID No: 95D-HQ-1334954 -3
2812 Fawkes Drive
Wilmington, Delaware 19808 LabNo.: 010223012 MQ BO
Reference: Your letter dated February 15, 2001
Your No.:
Title: UNSUB;
AMELIA EARHART - VICTIM;
MISSING PERSONS
Date specimens received: February IIe 2001
This report supplements and completes FBI Laboratory report dated May 7, 2001
(FBI Laboratory number 010223012 MQ BO). Please refer to that report for a complete
listing of the submitted items.
The following additional specimen was identified:
Ql1.1 Swab from button
The results of the mitochondrial DNA examinations are included in this
report. All requested examinations have been completed.
The remaining processed DNA from specimen Q1.1 is being returned under separate
cover. Specimen Q1 was returned to you by personal delivery on May 7, 2001. The
processed DNA can be found in a package marked "PROCESSED DNA SAMPLES:
SHOULD BE REFRIGERATED/FROZEN." It is recommended that these samples be stored
in a refrigerator/freezer and isolated from evidence that has not been examined.
Page 1 of 1
W0f2Y/ 6 /
-_ ,
This Report is Furnished for Official Use Only
oe)
Za\. LABORATORY las
FEDERAL BUREAU OF INVESTIGATION
WASHINGTON, D. C. 20535
Report of Examination
Examiner Name: Po Date: October 24, 2001
Unit DNA Analysis II Phone No.: 202-324-1265
CaseID No: 95D-HQ-1334954 LabNo. 010223012 MQ BO
Results of Examinations:
A mitochondrial DNA (mtDNA) sequence was obtained from the Q1.1 swab taken
from the Q1 button. The sequence from this sample showed the presence of multiple
ambiguous nucleotide positions and thus was not suitable for comparison purposes.
“ad ee Po ‘ =
DNAU II - Page 1 of 1
This Report is Furnished for Official Use Only